Figure 2.

The effect of performing only brightfield registration. (No post-processing drift correction was employed here). (a) Alpha tubulin in HeLa cells imaged without employing brightfield registration between datasets. (c) The same cell imaged with brightfield registration performed before the acquisition of each dataset. 6 datasets containing 2000 frames apiece were collected. (b, d) Zoomed in view of the selected region in the image to the left. All scale bars measure 1 $\mathrm{\mu }$m.