Fig. 4.

The differential effects of hinokitiol on iron chelation and mitochondrial respiratory activity between fungal cells and mammalian cells. (A-F) Mammalian BEAS-2B (A-C) and PANC-1 (D-F) cells were cultured with hinokitiol for 10 h. The cells were then stained with FeRhonox-1, fluorescence intensity was detected by flow cytometry (A, D), and the Gmean value was calculated (B, E). FeRhonox-1-stained cells were further observed by fluorescence microscopy (scale bars: 100 μm) (C, F). (G, H) C. albicans SC5314, mammalian BEAS-2B or PANC-1 cells were treated with different concentrations of hinokitiol for 12 h at 37 °C. CCK-8 assays were performed to reveal the effects of hinokitiol on mitochondrial dehydrogenase activities in fungal cells or mammalian cells. The detailed procedures were seen in Materials and methods section. The wells with addition of CCK-8 solutions were imaged by a camera (G), and the absorbance at 450 nm was measured by a microplate reader (H). The bars represent means ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.