TABLE 2.
Features of cytokinin two component signaling system genes in S. bicolor.
| Gene name | Gene symbol | Chr | Start site | End site | CDS | Exon | Introns | Protein length | Molecular weight(MW) | Isoelectric point(PI) | Instability index(II) | Aliphatic index | Grand average of hydropathicity (GRAVY) | Cell location |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Histidine kinases (HKs) | ||||||||||||||
| SbHK1 | LOC8059279 | 1 | 22266130 | 22269754 | 2451 | 5 | 4 | 743 | 8,0885.46 | 5.96 | 48.81-unst | 91.33 | −0.076 | Cytoplasmic |
| SbHK2 | LOC8075742 | 4 | 59266327 | 59273161 | 4149 | 10 | 9 | 973 | 108,350 | 5.91 | 38.85 | 87.58 | −0.179 | Plasma membrane |
| SbHK3 | LOC110433965 | 3 | 71489995 | 71495445 | 3709 | 11 | 10 | 1,005 | 112,373.2 | 8.42 | 38.35 | 90.22 | −0.226 | Extracellular |
| SbHK4 | LOC8060004 | 1 | 8870876 | 8878374 | 3669 | 11 | 10 | 996 | 109,153.9 | 5.51 | 46.05-unst | 91.66 | −0.09 | Cytoplasmic |
| SbHK5 | LOC8069360 | 10 | 55655919 | 55666166 | 4985 | 14 | 13 | 959 | 108,270.6 | 5.34 | 50.78-unst | 80 | −0.508 | Nuclear |
| SbCKl1 | LOC8076204 | 10 | 5136492 | 5152208 | 3457 | 8 | 7 | 1,138 | 122,065.4 | 5.63 | 42.59-unst | 91.31 | −0.055 | Plasma membrane, cytoplasmic, mitochondrial |
| SbERS1 | LOC8063415 | 1 | 9801882 | 9806982 | 2470 | 6 | 5 | 635 | 70,635.29 | 7.07 | 37.87 | 106.71 | 0.144 | Plasma membrane |
| SbETR1 | LOC8076011 | 9 | 5018113 | 5022906 | 2920 | 6 | 5 | 632 | 70,153.82 | 6.84 | 37.48 | 106.19 | 0.144 | Plasma membrane |
| SbEIN4.1 | LOC8155512 | 4 | 67810845 | 67814687 | 3633 | 3 | 2 | 899 | 99,732.68 | 8.28 | unst-50.1 | 100.46 | 0.078 | Plasma membrane |
| SbEIN4.2 | LOC8066019 | 6 | 3178416 | 3182964 | 3346 | 3 | 2 | 773 | 85,953.83 | 7.04 | 36.79 | 102.41 | 0.088 | Plasma membrane |
| SbPHYA | LOC8059991 | 1 | 8713754 | 8721151 | 3941 | 6 | 5 | 1,131 | 125,094.3 | 5.77 | unst-49.69 | 92.6 | −0.151 | Cytoplasmic |
| SbPHYB | LOC8081072 | 1 | 68035215 | 68043712 | 4750 | 4 | 3 | 1,178 | 129,046.6 | 5.75 | unst-48.61 | 86.72 | −0.167 | Cytoplasmic, plasma membrane |
| SbPHYC | LOC8086232 | 1 | 6748035 | 6753340 | 4292 | 4 | 3 | 1,135 | 126,205 | 5.72 | unst-51.31 | 97.24 | −0.112 | Cytoplasmic |
| Phosphotransfer Proteins (HPs) | ||||||||||||||
| SbHP1 | LOC8062536 | 2 | 19797592 | 19801117 | 1,132 | 6 | 5 | 145 | 16,228.54 | 4.55 | 40 | 91.52 | −0.079 | Nuclear, extracellular, cytoplasmic |
| SbHP2 | LOC8057993 | 7 | 60641495 | 60644235 | 824 | 6 | 5 | 144 | 16,172.66 | 5.63 | 26.55 | 88.68 | −0.089 | Nuclear, extracellular |
| SbHP3 | LOC8075559 | 3 | 62500906 | 62506524 | 962 | 7 | 6 | 151 | 17,252.52 | 7.58 | 56.36-unst | 57.62 | −0.645 | Nuclear |
| SbHP4 | LOC8068945 | 9 | 55183674 | 55186538 | 858 | 6 | 5 | 154 | 17,682.91 | 6.14 | 56.83-unst | 60.19 | −0.678 | Nuclear |
| SbHP5 | LOC8055697 | 9 | 8016183 | 8018505 | 900 | 5 | 4 | 151 | 17,800.24 | 8.42 | 60.23-unst | 72.32 | −0.754 | Nuclear |
| Response Regulators (RRs) | ||||||||||||||
| Type-A RRs | ||||||||||||||
| SbRR4 | LOC8077366 | 3 | 73177403 | 73181260 | 1873 | 5 | 4 | 245 | 26,365.79 | 5.24 | 51.26-unst | 83.63 | −0.41 | Nuclear |
| SbRR9 | LOC110435576 | 5 | 2740967 | 2743306 | 1,146 | 5 | 4 | 202 | 22,749.54 | 6.13 | 59.58-unst | 79.11 | −0.683 | Nuclear |
| SbRR10 | LOC8071408 | 8 | 1026753 | 1029205 | 1,304 | 5 | 4 | 279 | 30,788.82 | 6.56 | 69.03-unst | 83.55 | −0.352 | Nuclear |
| Type-B RRs | ||||||||||||||
| SbRR16 | LOC8056131 | 3 | 70434838 | 70438749 | 2055 | 5 | 4 | 579 | 64,700.22 | 5.04 | 45.88-unst | 81.3 | −0.424 | Nuclear |
| SbRR17 | LOC8079408 | 4 | 5416832 | 5421301 | 2579 | 6 | 5 | 631 | 68,631.82 | 6.12 | 39.97 | 77.4 | −0.533 | Nuclear |
| SbRR18 | LOC8084640 | 4 | 66411416 | 66416077 | 3155 | 6 | 5 | 675 | 73,136.19 | 5.96 | 46.1-unst | 80.99 | −0.341 | Nuclear |
| SbRR19 | LOC8056866 | 1 | 72774008 | 72779535 | 2724 | 6 | 5 | 686 | 73,679.76 | 6.26 | 49.94-unst | 77.76 | −0.423 | Nuclear |
| SbRR20 | LOC8076203 | 10 | 5132133 | 5136115 | 2344 | 6 | 5 | 672 | 72,886.35 | 6.01 | 46.69-unst | 80.45 | −0.331 | Nuclear |
| SbRR24.1 | LOC8058416 | 10 | 55120219 | 55130513 | 2088 | 8 | 7 | 695 | 76,440.93 | 5.56 | 43.44-unst | 74.46 | −0.459 | Nuclear |
| SbRR24.2 | LOC8084794 | 8 | 9453701 | 9488726 | 2163 | 9 | 8 | 551 | 60,852.22 | 5.66 | 36.22 | 80.69 | −0.536 | Nuclear |
| Type-C RRs | ||||||||||||||
| SbRR13 | LOC8083419 | 6 | 3752870 | 3753702 | 501 | 2 | 1 | 132 | 14,169.76 | 7.74 | 18.5 | 96.74 | 0.224 | Cytoplasmic |
| SbRR14 | LOC8085961 | 1 | 7312249 | 7313602 | 963 | 4 | 3 | 201 | 21,501.8 | 9.69 | 54.67-unst | 88.41 | −0.106 | Chloroplast |
| Pseudo-RRs | ||||||||||||||
| SbPRR1 | LOC8072479 | 4 | 56625637 | 56628735 | 2328 | 6 | 5 | 524 | 58,166 | 5.91 | 52.76-unst | 66.37 | −0.623 | Nuclear |
| SbPRR2.1 | LOC8078076 | 3 | 302694 | 310420 | 2682 | 6 | 5 | 395 | 42,195.43 | 6.06 | 56.94-unst | 68.3 | −0.385 | Nuclear |
| SbPRR2.2 | LOC8057890 | 10 | 8684841 | 8687455 | 1952 | 6 | 5 | 466 | 50,071.47 | 5.01 | 56.48-unst | 68 | −0.438 | Nuclear |
| SbPRR3.1 | LOC8070845 | 6 | 40305107 | 40316802 | 3093 | 10 | 9 | 291 | 31,977.47 | 5.08 | 55.20-unst | 70.34 | −0.646 | Nuclear |
| SbPRR3.2 | LOC8084889 | 1 | 69433326 | 69440358 | 3195 | 12 | 11 | 765 | 83,595.91 | 6 | 52.17-unst | 59.52 | −0.88 | Nuclear |
| SbPRR5 | LOC110432809 | 2 | 65784791 | 65790044 | 2553 | 8 | 7 | 630 | 70,207.3 | 6.2 | 52.28-unst | 65.3 | −0.827 | Nuclear |
| SbPRR9 | LOC8058579 | 5 | 4190668 | 4195535 | 2582 | 8 | 7 | 697 | 75,487.93 | 7.32 | 56.89-unst | 62.48 | −0.716 | Nuclear |