Regulation of CE on LTCC by Cardio ECR and patch-clamp. (a) The impedance and field potential of hiPSC-CMs were real-time monitored after Bay-K-8644 treatment (n = 5 per group). (b) I–V curve (left) of Ca current and representative Ca current traces (right) recorded from the control group, Bay-K-8644 group, CE + Bay-K-8644 group, and nisoldipine + Bay-K-8644 group in hiPSC-CMs (n = 5 per group). The data were expressed as the mean ± SD. #P < 0. 05 vs control group (The color of # was the same as the color of the curve); *P < 0.05 vs Bay-K-8644 group (The color of * was same as the color of the curve). (c) After incubating CE for 24 h, hiPSC-CMs were hypoxic, and the impedance and field potential were monitored during reoxygenation 24 h (n = 5 per group). (d) After rat MI/R injury, NRVMs were obtained. Moreover, I–V curve (left) of Ca current and representative Ca current traces (right) recorded from the sham group, IR group, and CE + IR group. The data were expressed as the mean ± SD. #P < 0. 05 vs control (sham) group (The color of # was same as the color of the curve); *P < 0.05 vs HR (IR) group (The color of * was same as the color of the curve).