Fig. 3.

TA interacts with SHP-2 directly. (A, B) U2OS cell lysates were incubated in the presence or absence of TA for 1 h at room temperature, followed by proteolysis with indicated ratios of pronase for 20 min, the lysates were analyzed by Western blotting (A) The SDS-PAGE gels were stained by coomassie blue. (B) The content of SHP-2 was analyzed by SHP-2 antibody, *P < 0.05, **P < 0.01, ***P < 0.001 versus control. (C) The stabilizing effects of TA on SHP-2 at increasing temperature up to 64 °C were analyzed by Western blotting. (D) TA increased the thermal stability of SHP-2 compared with DMSO-treated U2OS cell lysates. U2OS lysates were mixed with the indicated doses of TA at 50 or 37 °C to evaluate the thermal stability of SHP-2.