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. 2021 Jan 23;34:137–147. doi: 10.1016/j.jare.2021.01.009

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© 2021 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 5 — Effects of Nar on mitochondrial dysfunction, histomorphology and in vivo AMPKα/Sirt1 signaling pathway. (A) Concentration of cytochrome c level in mitochondria or cytoplasm in different groups. (B) Mitochondrial membrane potential in different groups. (Scale bar: 33 μm). (C) Concentration of Hcy in rat plasma after H-Met fed for 4 weeks. (D) The expression level of SOD in different groups. (E) The concentration of NO in rat’s plasma. (F) The concentration of MDA in rat’s plasma. (G) Histopathology of thoracic aortas in control group. (H) Histopathology of thoracic aortas in H-Met group. (I) Histopathology of thoracic aortas in Nar group. Distinct histopathological characteristic in each group are indicated by arrowhead. (Scale bar = 10 μm, Magnification: 200×). (J) Western blotting analysis of AMPKα, Sirt 1 and eNOS in different groups. (K) The relative expression of AMPKα in different group. (L) The relative expression of Sirt 1 in different group. (M) The relative expression of eNOS in different group.