Figure 1.

Differential expression profiles of circRNA and ceRNA networks in URSA and normal pregnancy

(A) Scatterplot of differential circRNA expression in placental villus of patients with normal pregnancy and URSA in early pregnancy. (B) Volcano plot shows the different circRNA expression in these two groups. (C) Hierarchical cluster analysis of upregulated and downregulated circRNAs in four pairs of normal pregnancy and URSA placental villus; red is higher expression and blue is lower expression. (D) Number of differentially expressed circRNAs in different chromosomes. (E) Flowchart of the steps used to build the ceRNA network in URSA villus. (F) Characterization of five top circRNAs in the ceRNA network. (G) Validation of microarray results by qRT-PCR analysis of top five circRNAs in the ceRNA network (circRNA-0050703, circRNA-0046770, circRNA-0042563, circRNA-0042569, circRNA-0046769) in four pair cases. Three different independent experiments with three technical repetitions were performed. Data are expressed as the mean ± SEM. ∗p < 0.05. NS, not significant.