Fig. 2.

ATPase activity of PEDV nsp13. (A) Detection of the ATPase activity of PEDV nsp13 using the Kinase-Glo Plus Luminescent Kinase Assay kit. (B) Purified nsp13 recombinant proteins (0, 0.1, 0.2, 0.5, 1, 2, or 5 μM) were incubated with ATP in reaction buffer at 37℃ for 10 min, then Kinase-Glo reagent mix was added, and the ATPase activity was measured. (C) PEDV nsp13 (0.2 μM) was reacted with ATP at 37℃ for 5, 10, 20, 30, 40, or 50 min., then the ATPase activity was measured. (D) PEDV nsp13 (0.2 μM) was incubated in reaction buffer in the presence of MgCl₂, MnCl₂, CuCl₂, or ZnCl₂, then the ATPase activity was measured. (E, F) PEDV nsp13 (0.2 μM) was incubated in reaction buffer containing the indicated concentrations of MgCl₂(E) or MnCl₂(F), then the ATPase activity was measured.