FIG. 2.

Expression analysis of YGR transgenic mice. (A) Determination of the YAC copy number in YGR mice by Southern blot analysis of tail DNA from WT (wt) mice and one transgenic mouse from each generation (F₁ and F₂). (B) Analysis of transgene expression in the hippocampus of a C57BL/6 mouse, an FVB/N mouse, and a YGR mouse by RT-PCR and RFLP. PCR products were cut with RsaI and analyzed by Southern blotting. (C) Analysis of transgene expression in various tissues by RT-PCR and RFLP. br, whole brain; hyp, hypothalamus; co, cortex; pit, pituitary; ad, adrenal; thy, thymus; sp, spleen; li, liver; lu, lung; w, wild type; Y, YGR transgenic mice. (D) Western blot analysis of GR protein expression in the hippocampus. wt, wild type.