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. 2021 Dec 7;41(12):BSR20211201. doi: 10.1042/BSR20211201

Figure 1. Lung ACE and ACE2 mRNA and protein expression in male and female normotensive (Wistar) and hypertensive (SHR) rats.

Figure 1

The gene expression of (A) ACE and (B) ACE2 was quantitatively measured by RT-PCR and normalized to cyclophilin and is expressed as the % of male Wistar rats. (C) Lung ACE/ACE2 gene expression ratio was expressed as 2−ΔCt/2−ΔCt. The protein expression of (D) ACE and (E) ACE2 was determined by immunoblotting of lung membrane proteins and normalized to β-actin and is expressed as the % of male Wistar rats (Supplementary Figures S3 and S4). The values represent individual measurements and the means ± SEMs. *P<0.05; **P<0.01; ***P<0.001.