Figure 4. Lung ADAM17 activity and its correlation with lung ACE2 protein and serum abundance in male and female normotensive (Wistar) and hypertensive (SHR) rats.

(A) Lung ADAM17 activity was determined by a fluorescent enzymatic assay in the presence or absence of TAPI 0. The TAPI 0 sensitive enzymatic activity was expressed as RFUs/µg/h. The values represent individual measurements and the means ± SEMs. The absence of a correlation between lung ADAM17 activity and (B) lung ACE2 protein or (C) serum ACE2 concentration. The correlation coefficients (r) and P-values were obtained by Pearson’s correlation test, and lines were obtained by linear regression plotting.