Figure 2.

Anti-CD123.2B4.ζ CAR-NKs have transient anti-AML activity in vivo. (A) Schematic of MV-4-11 xenograft model. On D0, NSG mice were injected via tail vein with 1×10⁶ CD123(+) MV-4-11 cells that express ffLuc (MV-4-11.ffLuc cells). In treatment groups, 10×10⁶ NK cells were administered on D7. Cohorts: UTD/unmodified, 4-1BB.ζ CAR-NK, and 2B4.ζ CAR-NK. (B) Leukemia proliferation was monitored with bioluminescence imaging and was recorded as photons/s/cm²/sr; n=8–12 mice per group. Magnification of D7–D21 shown. (C) Representative images of three mice per condition. Minimum and maximum values of color scale are depicted at the top (min–max). (D) Kaplan-Meier survival analysis of MV-4-11 xenografts (n=8–12 mice per condition). (E) Mouse PB collected at indicated time points and analyzed via flow cytometry. Each dot represents a single mouse. Solid line: median. At later time points, NK-cell count was undetectable for all groups and is not plotted. *P<0.05, **P<0.01, ****P<0.0001. AML, acute myelogenous leukemia; CAR, chimeric antigen receptor; D, day; ffLuc, firefly luciferase; NK, natural killer; PB, peripheral blood; UTD, untransduced.