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. Author manuscript; available in PMC: 2021 Dec 9.
Published in final edited form as: Anal Chem. 2021 Mar 16;93(12):5054–5060. doi: 10.1021/acs.analchem.0c04292

Figure 3.

Figure 3.

Extracted ion chromatograms of hydrophilic peptides from a trypsin digestion of 2 pmol of tissue factor. Peptides that were not retained on the C8 trap and only weakly retained on the PGC column are region 1 (blue): (A) DVK, (B) SSSSGK, and (C) SSSSGKK. Peptides that were well retained by the PGC column and eluted from the column during the PGC gradient are region 3 (blue): (D) TLVR, (E) STNFK, and (F) DVFGK. One peptide was split between the PGC and C18 columns due to some retention on the C8 trap, region 2 (red): (G) QTYLAR.