FIG. 4.

Subcellular localization of the alternatively spliced Smad4 variants in the absence and presence of LMB. NIH 3T3 cells were transfected with Flag-tagged Smad4 isoforms as indicated. (Left) The subcellular localization of the Smad4 isoforms in untreated cells was determined by indirect immunofluorescence using the anti-Flag antibody, and nuclei of the same cells were also stained with DAPI as indicated. (Right) Subcellular localization of the Smad4 isoforms in cells treated with LMB at a 20-ng/ml final concentration for 1 h. wt, wild type.