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. 2021 Nov 25;9:757184. doi: 10.3389/fcell.2021.757184

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Copyright © 2021 Zhou, Wang, Ding, Yang and Xie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of ATG5 in cervical cancer cell lines (A) The expression of ATG5 mRNA was detected in Hela and Ca Ski (B) After 72 h infection, Hela and Ca Ski cells with green fluorescence were the cells that transfected plasmid successfully. Magnification ×100 (C) mRNA expression of ATG5 was significantly inhibited by ATG5-RNAi compared with the control group. Error bars represent ±SD. *p < 0.05, **p < 0.01, ***p < 0.001 (D) Protein expression of ATG5 could be detected in Hela and Ca Ski, and was significantly downregulated by ATG5-RNAi in Hela-ATG5-KD and Ca Ski-ATG5-KD cells compared with the control group. PANC-1 was used as a positive control.