| scan type |
SRM |
| source |
H-ESI |
| ion polarity |
negative |
| vaporizer temperature (°C) |
300 |
| ion transfer tube temperature (°C) |
250 |
| sheath gas (arbitrary scale) |
30 |
| auxiliary gas (arbitrary scale) |
10 |
| CID gas (mTorr) |
1.5 |
| dwell time per transition (ms) |
148 |
| RF lens for rottlerin (V) |
159 |
| RF lens for IS (V) |
125 |
| Q1 resolution (FWHM) |
0.7 |
| Q3 resolution (FWHM) |
0.7 |
| collision energy for rottlerin (V) |
24 |
| collision energy for IS (V) |
20 |
| ion transition for rottlerin (m/z) |
515.2 → 321.2 |
| ion
transition for IS (m/z) |
168.0→ 132.1 |
