Table 2. LC Conditions for Quantification of Rottlerin in Plasma.

parameters	conditions
column	Purospher STAR RP-18 (50 × 4.6 mm, 5 μm)
elution	isocratic
mobile phase	10 mM ammonium formate buffer/acetonitrile: 5:95 (%, v/v)
flow rate	0.3 mL/min
column temperature	30 °C
autosampler temperature	4 °C
retention time of rottlerin	0.8 min
retention time (IS)	0.7 min
run time	2.5 min