FIG. 2.

DSB repair products. Restriction maps of parental (A) and repaired neo⁺ alleles (B). NcoI digestion is diagnostic for homologous repair of the I-SceI-generated DSB in either 5′neo allele. BglII/SacII digestion is used to characterize the type of recombination event. BglII/SacII fragments of variable size (B) indicate gene conversion tracts incorporating variable lengths of chr.14 sequences (+chr.14) and NHEJ at variable positions of chr.17. BII, BglII; N, NcoI; SII, SacII. (C) Southern blot analysis of representative clones. Note that the 3′neo allele is unchanged from the parental allele in each of the recombinant clones. The probe was a 5′neo gene fragment. (D) Position of PCR primers derived from chr.17 sequences that flank the repair junctions.