FIG. 3.

Sequence analysis of repair junctions from class II neo⁺ clones. PCR products from the neo⁺ allele of the Fneo⁺/3′ (A) and Rneo⁺/3′ cell lines (B) were cloned and sequenced. Extension on chr.14 indicates the length of newly synthesized DNA incorporated at one end of the chr.17 break site beyond the neo stop codon. Thus, the total extension includes an additional 229 bp to the number indicated. Deletion on chr.17 indicates the number of base pairs deleted from the nonconverted end of the I-SceI break site prior to NHEJ. The sequences at the junctions of chr.14 and chr.17 are separated by a hyphen when there is no sequence overlap, underlined if microhomology is present, or indicated by the base pairs that were inserted at the junction.