TABLE 2.
Repair classes of DSB-induced interchromosomal recombination
| Repair class | No. of clones
|
|
|---|---|---|
| F5′/3′ | R5′/3′ | |
| Gene conversion without an exchange | ||
| I (conversion tract, <0.2 kb)a | 0 | 0 |
| II (conversion tract, <1.0 kb) | 29 | 24 |
| III (conversion tract, <3.2 kb) | 4 | 1 |
| IV (conversion tract, >3.2 kb) | 12 | 9 |
| Translocation | 0 | 1b |
| Total | 45 | 35 |
a
Gene conversion tracts of less than 229 bp do not produce a functional neo+ gene, and thus these products are not recovered.
b
The translocation was between chr.17 and an unidentified chromosome(s) (see Fig. 4B).