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Intracellular reactive oxygen species validated using highly fluorescent DCF in F. diplosiphon B481-WT and B481-SD strains grown in BG-11/HEPES medium supplemented with 1.6, 3.2, 6.4, 12.8, and 25.6 mg L–1 nZVIs. Average DCF fluorescence (±standard error) of three biological replicates for each sample is shown. Different letters above the error bars indicate significance among treatments (p < 0.01).
