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. 2021 Dec 9;40:386. doi: 10.1186/s13046-021-02194-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — The biological function of miR-130b/301b and USP13 in vitro. Cell proliferative capacity was measured by Cell Counting-Kit 8 (CCK-8) (a and b) assay and colony formation assay (e) in miR-130b/301b overexpressed 5637 and UM-UC-3 cells. CCK-8 (c and d) and colony formation assay (f) were also performed to evaluate the cellular proliferation in USP13 knocked down 5637 and UM-UC-3 cells. Cell invasive and migrative capacities were measured by transwell assay in miR-130b/301b overexpressed (g and h) or USP13 knocked down (i and j) 5637 and UM-UC-3 cells. Cell proliferation was detected by CCK-8 (k) and colony formation assay (l) in USP13 knocked down alone or USP13 knocked down as well as PTEN expression restored 5637 and UM-UC-3 cells. Cell invasive (m) and migrative (n) capacities were measured by Transwell assay in USP13 knocked down alone or USP13 knocked down as well as PTEN expression restored 5637 and UM-UC-3 cells. Original magnification: 400×. *P < 0.05 and **P < 0.01, as determined by Student’s T-test