FIG. 4.

The association of hnRNPs C1 and C2 with TRs is conserved. (A) hTR and mTR were incubated in VA13-hTERT S100 extracts, exposed to UV irradiation, treated with RNase, and run on an acrylamide gel containing SDS. (B) Full-length mTR was incubated with the indicated oligonucleotide at 37°C prior to the addition of VA13-hTERT S100 extract. Following incubation with the oligonucleotide and extracts, the reaction was exposed to UV irradiation, RNase treated, and run on an acrylamide gel containing SDS. A variant of mTR lacking the 4-base uridylate tract next to the template and wild-type mTR were incubated in S100 extracts derived from VA13-hTERT cells, exposed to UV irradiation, RNase treated, and run on an acrylamide gel containing SDS.