FIG. 6.

The binding of hnRNP C proteins to telomerase correlates with the ability of telomerase to function on the telomere and can colocalize with telomeric bound TRF1 and TRF2. (A) Extracts from VA13 cells expressing the C-terminal HA-tagged hTERT and wild-type hTR were mixed with protein G agarose beads (No Ab) or protein G agarose beads precoupled to antibodies against hTERT, hnRNP C, or an irrelevant protein (antibody RGF against elongation factor [RF]); were incubated at 4°C for 1 h; and were washed extensively with cell lysis buffer and assayed for telomerase activity using the TRAP reaction. The lane labeled Input represents 5% of the amount of cells used for the immunoprecipitation. (B) Indirect immunofluorescence analysis of fixed HT1080 cells grown on chamber slides with anti-hnRNP C antibody (green) and anti-TRF1 and anti-TRF2 antibody (red). The merger of the red and green images yields yellow, which indicates colocalization.