| BD Insulin syringe 0.5 mL |
Thermo Fisher Scientific |
14-826-79 |
|
| Pentobarbital |
Millipore Sigma |
P3761 |
|
| Trypsin EDTA 0.05% |
Thermo Fisher Scientific |
25300-062 |
|
| Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F12) |
Thermo Fisher Scientific |
11330-057 |
|
| Antibiotic-Antimycotic (100X) |
Thermo Fisher Scientific |
15240062 |
Pen/Strep also works |
| DMEM/F12, no phenol red |
Thermo Fisher Scientific |
11039-021 |
|
| Dulbecco’s phosphate-buffered saline (DPBS) |
Thermo Fisher Scientific |
14190-250 |
Without Mg2+/Ca2+ |
| Matrigel™ Growth Factor Reduced (GFR); Phenol Red-Free; 10 mL |
Fisher Scientific |
CB-40230C |
Lot: 8204010, stock concentration 8.9 mg/mL |
| DNase (Deoxyribonuclease I) |
Worthington Biochemical |
LS002007 |
|
| Millicell EZ SLIDE 8-well glass, sterile |
Millipore Sigma |
PEZGS0816 |
These chamber slides are great for gasket removal but other brands can work well (e.g. Lab Tek II). |
| Class 3 Collagenase |
Worthington Biochemical |
LS004206 |
|
| Class 2 Dispase (Roche) |
Millipore Sigma |
4942078001 |
|
| Fetal Bovine Serum |
VWR |
97068-085 |
100% US Origin, premium grade, Lot: 059B18 |
| MillexGV Filter Unit 0.22μm |
Millipore Sigma |
SLGV033RS |
|
| Gentamicin |
Thermo Fisher Scientific |
15710064 |
|
| Insulin |
Millipore Sigma |
I6634-100mg |
|
| EGF |
Fisher Scientific |
AF-100-15-100ug |
|
| N-2 Supplement (100x) |
Thermo Fisher Scientific |
17502048 |
|
| B27 supplement without vitamin A (50x) |
Thermo Fisher Scientific |
12587010 |
|
| Nrg1 |
R&D |
5898-NR-050 |
|
| R-spondin |
Peprotech |
120-38 |
|
| Rho inhibitor Y-27632 |
Tocris |
1254 |
|
| 70μM nylon cell strainer (Corning) |
Fisher Scientific |
08-771-2 |
|
| 15 ml High-Clarity Polypropylene Conical Tube (BD Falcon) |
Fisher Scientific |
352096 |
|
| 50 ml High-Clarity polypropylene conical tube (BD Falcon) |
Fisher Scientific |
352098 |
|
| 35 mm TC-treated Easy-Grip Style Cell Culture Dish (BD Falcon) |
Fisher Scientific |
353001 |
|
| 60 mm TC-treated Easy-Grip Style Cell Culture Dish (BD Falcon) |
Fisher Scientific |
353004 |
|
| Corning® Costar® Ultra-Low Attachment 6-well |
Fisher Scientific |
CLS3471 |
|
| Ovine Pituitary Prolactin |
National Hormone and Peptide Program |
|
Purchased from Dr. Parlow at Harbor-UCLA Research and Education Institute |
| Dexamethasone |
Millipore Sigma |
D4902-25MG |
|
| Fluoromount-G (Southern Biotech) |
Fisher Scientific |
0100-01 |
Referred to as mounting media in text |
| Corning Cell Recovery solution |
Fisher Scientific |
354253 |
Follow the guidelines for use – Extraction of Three-Dimensional Structures from Corning® Matrigel® Matrix |
| Paraformaldahyde |
Millipore Sigma |
PX0055-3 |
|
| Sterile Filtered Donkey Serum |
Equitech-Bio Inc. |
SD30-0500 |
|
| Triton X-100 |
Millipore Sigma |
x100-500ML |
Laboratory grade |
| NaCl |
Fisher Scientific |
S671-3 |
|
| KCl |
Fisher Scientific |
P217-500 |
|
| NaH2PO4 |
Fisher Scientific |
S468-500 |
|
| KH2PO4 |
Fisher Scientific |
P285-500 |
|
| Sterile Filtered Donkey Serum |
Equitech-Bio Inc. |
SD30-0500 |
|
| Sodium Hydroxide |
Fisher Scientific |
S318-500 |
|
| Glycine |
Fisher Scientific |
BP381-5 |
|
| 24 well ultra–low attachment plate (Corning) |
Fisher Scientific |
CLS3473-24EA |
|
| KRT14–CreERtam |
The Jackson Laboratory |
5107 |
|
| R26R-EYFP |
The Jackson Laboratory |
6148 |
|
| B6 ACTb-EGFP mice |
The Jackson Laboratory |
003291 |
|
| Mouse anti-SMA |
Millipore Sigma |
A2547 |
Use at 1:500, Lot: 128M4881V, stock 5.2 mg/mL, RRID:AB_476701 |
| Donkey anti-Mouse 647 |
Jackson ImmunoResearch |
715-606-150 |
Use at 1:1000, Lot: 140554, stock 1.4 mg/mL |
| Goat anti-WAP |
Santa Cruz Biotech |
SC-14832 |
Use at 1:250, Lot: J1011, stock 200 μg/mL, RRID:AB_677601 |
| Donkey anti-Goat 647 |
Thermo Fisher Scientific |
A21447 |
Use at 1:500, Lot: 1608641, stock 2 mg/mL, RRID:AB_2535864 |
| Hoechst 33342 |
AnaSpec |
AS-83218 |
Use 1:2000, stock is 20mM |
