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Distribution of Rab3A in MCF-7 cells. (A) Cytosol (Cyto.) and membrane (Mem.) fractions from control (−) and HRG-treated (+) (30 ng/ml, 8 h) cells were analyzed by Western blotting. (B) Postnuclear supernatants from control (−) and HRG-treated (+) (8 h) cells were extracted with Triton X-114 as described (7). The levels of Rab3A present in aqueous (lanes 1 and 2) and detergent (lanes 3 and 4) phases were determined by Western blotting.
