Table 2.

Ka/Ks analysis of TPS genes in C. faberi.

Gene pairs	Ka1	Ks2	Ka/Ks3 Ratio	Date (Mya)
CfTPS5-CfTPS12	0.036349	0.060903	0.596841353	3.346319222
CfTPS18-CfTPS22	0.183699	0.58801	0.31240786	32.30822426
CfTPS27-CfTPS28	0.061668	0.086265	0.71486064	4.739845716
CfTPS15-CfTPS23	0.040242	0.038395	1.048087536	2.109640277
CfTPS3-CfTPS10	0.329855	1.100284	0.299790513	60.45518349
CfTPS20-CfTPS7	0.035647	0.054369	0.655636934	2.987330286
CfTPS25-CfTPS8	0.052051	0.057777	0.900881064	3.174578759
CfTPS1-CfTPS2	0	0.01373	0	0.754415417
CfTPS13-CfTPS16	0.207258	0.585853	0.353772093	32.1897194
CfTPS11-CfTPS29	0.108769	0.203168	0.535365599	11.16305388
CfTPS14-CfTPS30	0.035698	0.0446	0.800403031	2.450539375
CfTPS24-CfTPS32	0.028484	0.045777	0.622244514	2.515215271
CfTPS21-CfTPS26	0.04343	0.044138	0.983976748	2.425138016

¹Ka, non-synonymous rate.

²Ks, synonymous substitution.

³Ka/Ks, evolutionary constraint.

Divergence time (T) was calculated by using the formula T=Ks/(2×9.1×10⁻⁹)×10⁻⁶ million years ago (Mya, Zhang et al., 2018). The details of Ka/Ks calculation are listed in Supplementary Table S8.