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. 2021 Nov 25;12:700933. doi: 10.3389/fimmu.2021.700933

Figure 7.

Figure 7

Small RNA sequencing revealed that miR-221-5p is upregulated in response to LPS in vitro, resulting in JNK2 downregulation. mRNA expressions of JNK2 in LPS- (A) or PA103-treated (B) RAW 264.7 cells, or LPS-treated THP-1 cells (PMA-differentiated) (C) as indicated. (D) Heatmap of differential expressed miRNAs in RAW 264.7 cells treated with LPS for 0, 4, and 8h. (E) Seed sequence of miR-221-5p matching to JNK2 mRNA 3’UTR. (F) Levels of miR-221-5p in RAW 264.7 cells treated with LPS for different times. (G) JNK2 mRNA levels in mouse lung epithelial cells (MLE-12) treated with mimic control or miR-221-5p mimic (left panel), or inhibitor control or miR-221-5p inhibitor (right panel). (H) JNK2 mRNA levels in whole lung homogenates of mice intratracheally treated without or with miRNA inhibitor control or miR-221-5p inhibitor. (I) JNK2 3’UTR luciferase activity in the presence of miR-221-5p mimic (left panel; normalized to control mimic) or miR-221-5p inhibitor (right panel; normalized to control inhibitor). (J) JNK2 mRNA in the RNA-protein complex using Ago2 or control IgG for IP in the presence of miR-221-5p mimic (normalized to input). Data are presented as means ± sem. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant.