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The effect of the amount of quencher DNA (BHQ2- DNA) on the detection performance of nanobiosensor.
| Complementary or non-complementary (μL) c | Quencher DNA (μL) c | QDs-DNA nanoprobe (μL) b | RNase-free water (μL)) | Samplea |
| Xd (0, 2, 5, 9, 15) | 5 | 5 | 170 - X | 1 |
| 8 | 5 | 167 - X | 2 | |
| 3 | 5 | 172 - X | 3 |
a) Total volume is 180 μL; b) ʎex = 325 nm and ʎem range for FL measurments = 460–640 nm; c) the concentrations were 0.6 µM and d) X = 0–15 μL.
