Table 4.

Biopsy requirements

Forms required Equipment	Before performing an embryo biopsy, it is mandatory to fill form F and G in duplicate
	Inverted microscope with light field objectives (4×, 10×, 20×) and Hoffman objective (40×) with heated stage
	Holding micromanipulator and microinjector
	Biopsy micromanipulator and microinjector
	Laser shot system (mandatory)
	Laminar flow hood incorporated with UV light (mandatory)**
	Laminar hood in different rooms (advisable)**
	Stereo Zoom microscope for tubing
	Deep freezer (−20°C) (mandatory)
	Minicentrifuge (6000 rpm)
	Micropipettes with adjustable volumes (0.5-10 µL; 20-200 µL; 100-1000 µL)
Consumables/disposables	Biopsy and holding micropipettes for embryo biopsy
	Flexipets/denudation pipettes/glass capillaries for embryo and biopsy manipulation
	Barrier tips for micropipettes: Sterile tips, with white filter; RNase, DNase, pyrogen, and DNA free (should fit your micropipettes 0.5-10 µL and 20-200 µL or 100-1000 µL)
	Use surface decontaminant like ethanol
	Sterile disposable surgical gown (mandatory)
	Sterile latex/nitrile gloves (nonpowdered only)
Medium	The use of standard IVF culture medium during biopsy is “acceptable,” but its effectiveness may be highly dependent upon the developmental stage of the embryo biopsied

IVF=In vitro fertilization, UV=Ultraviolet