Fig. 7.

Therapeutic administration of DON decreases pro-inflammatory T cells and enhances anti-inflammatory T cells in the DLN.C57BL/6 mice were infected with HSV-1 and DON (0.3 mg/kg) was administered from day 6 until 15 PI and FACS analysis was performed on DLN at day 15 PI. Histograms and plot analysis present the frequencies (A and B) and numbers (C) of total CD4⁺ T cells, frequencies (D and E) and numbers (F) of Th1 cells (CD4⁺IFN-γ), frequencies (G and H) and numbers (I) of Th17 (CD4⁺IL-17A⁺) cells and frequency (J and K) and numbers (L) of Treg cells. The ratios of Treg:Th1 (M) and Treg:Th17 (N) in the DLN of control and DON treated animals were also presented. The data represent mean results ± SEM. All data were analysed by Mann-Whitney U test (experiments repeated three times with n=6).