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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Cell Immunol. 2021 Oct 14;370:104450. doi: 10.1016/j.cellimm.2021.104450

Fig. 8.

Fig. 8.

Effect of glutamine metabolism on in-vitro induction of Th1, Th17 and Treg cells. Splenocytes from DO11.10 RAG2−/− mice were ex-vivo cultured with cytokines promoting Th1, Th17 and Treg cell proliferation and tested under different concentration of glutamine and DON. After five days of incubation, the cells were harvested and analysed for the differentiation of the targeted immune phenotypes. The histogram A shows the frequencies of Th1 (CD4+IFN-γ+) cells with media containing 0, 1, and 2 mM concentrations of glutamine. Histogram B shows the frequencies of cells expressing IFN-γ at varying concentrations of DON. The histograms C and D and E and F show the same data for Th17 and Treg, respectively. The data represent mean results ± SEM. Dunnett's multiple comparisons test were performed as post-hoc test following the one-way ANOVA (experiments repeated three times with n=3).