Fig. 9.

DON treatment reduces the viral titer from latently infected TG ex-plant culture. BALB/c mice were infected with HSV-1 KOS EGFP/RFP and the TG of said mice were then extracted after day 34 PI. The effect of 5 μM DON was tested on ex-vivo reactivation assay alongside with 0.5 mM of 2DG and culture control media. The 2DG treatment functioned as a positive control while the control culture media was a mock control. The supernatant was removed daily and the virus titer was counted on Vero cells in a plaque assay. Data analysis were performed on transformed [Y=Log(Y)] viral titer values. Dunnett's multiple comparisons test were performed as post-hoc test following the two-way ANOVA (experiments repeated three times with n=3). The data represent mean results ± SEM.