KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
|
| ||
| Antibodies | ||
|
| ||
| CD45.2 | Biolegend | Clone: 104; Cat# 109820; 109806; 109814 |
| CD45.1 | Biolegend | Clone: A20; Cat#110722; 110706; 110708; 110714 |
| CD3e | Biolegend, BioxCell | Clone: CD3e (145–2C11); Cat#100204; BE0001–1 |
| TCRβ | Biolegend | Clone: H57–597;Cat#109208 |
| CD8α | Biolegend; eBioscience | Clone: 53–6.7; Cat# 100744; 100734; Cat #45–0081-82 |
| CD4 | Biolegend | Clone:RM4.5; Cat#100528 |
| Thy1.2 | Biolegend | Clone: 30-H12; 105326 |
| CD11b | Biolegend | Clone:M1/70; Cat#101263 |
| H2-Db | Biolegend | Clone:KH95; Cat# 111507 |
| H2-Kb | Biolegend | Clone: AF6–88.5; Cat#116518 |
| I-Ab/I-Eb | eBioscience | Clone: (M5/114.15.2); Cat#17–5958-82 |
| PD-1 | eBioscience | Clone: RMP1–30; Cat#25–5982-82 |
| TIM3 | Biolegend | Clone: RMT3–23; Cat#119704 |
| LAG-3 | Biolegend; BioXcell | Clone: C9B7W; Cat#125210; BE0174 |
| TIGIT | eBioscience | Clone:GIGD7;Cat# 12–9501-82 |
| TNFα | eBioscience | Clone:Mp6-XT22; Cat#12–7321-82 |
| IFNγ | BD | Clone: XMG1.2; Cat#554413 |
| Granzyme B | Biolegend | Clone: QA16A02; Cat#372211 |
| B220 | Biolegend | Clone:RA3–6B2; Cat#103206 |
| CD44 | Biolegend | Clone: IM7; Cat#103030 |
| CD62L | Biolegend | Clone: Mel-14; |
| CD69 | Biolegend | Clone: H1.2F3; |
| CD11c | Biolegend | Clone: HL3; |
| Eomes | Biolegend | Clone: Dan11mag; |
| T-bet | Biolegend | Clone: 4B10; |
| Egr2 | Biolegend | Clone: erongr2; |
| TOX | Miltenyi | Clone: REA473; Cat#130–118-474 |
| CD28 | BioXCell | Clone: PV-1; Cat#BE0015–5 |
| CD16/32 | Bio X Cell | 2.4g2;Cat# BE0307 |
|
| ||
| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| PMA | Sigma | Cat# P1585 |
| ionomycin | Sigma | Cat# I0634 |
| CellTrace Violet Cell Proliferation Kit, for flow cytometry | Invitrogen | Cat# C34557 |
| APC BrDU Kit | BD | Cat# 552598 |
| Foxp3 staining kit | eBioscience | Cat#00–5523–00 |
| LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit | Invitrogen | Cat#L10119 |
| 1X DPBS GIBCO 14190–250 | GIBCO 14190–250 | Cat#14190–250 |
| RPMI 1640 | GIBCO | Cat#11875–119 |
| DMEM | GIBCO | Cat#11965–118 |
| Fetal Bovine Serum | Gemini | Cat#100–106 |
| Penicillin/Streptimycin | GIBCO | Cat#15140–122 |
| 0.05% Trypsin EDTA | Cat#25300062 | |
| Dnase I | Roche | Cat#10104159001 |
| Collgenase IV | Sigma | Cat#C5138 |
| IL-2 | Biolegend | Cat#575402 |
| IL-7 | Peprotech | Cat#217–17 |
| IL-15 | Biolegend | Cat#566301 |
| GM-CSF | Biolegend | Cat#576304 |
| CD8a Microbeads (mouse) | Miltenyi | Cat#130–117–044 |
| Venor GeM Mycoplasma Detection Kit, PCR-based | Sigma | Cat#MP0025–1KT |
|
| ||
| Experimental models: Cell lines | ||
|
| ||
| C1498 | ATCC | Cat#TIB-49 |
| C1498.SIY | This lab | Zhang et al., 2009 |
| C1498.B7.1 | This paper | N/A |
| B16.F10 | A gift from Thomas Gajewski, University of Chicago | N/A |
| EL4 | A gift from Thomas Gajewski, University of Chicago | N/A |
| C1498.H2-Kb−/− | This lab | Kline et al., 2018 |
| C1498.H2-Db−/− | This paper | N/A |
|
| ||
| Experimental models: Organisms/strains | ||
|
| ||
| C57BL/6 | Taconic | Cat# B6 |
| B6.SJL-Ptprca/BoyAiTac | Taconic | Cat# 4007 |
| Rag2−/− | Taconic | Cat# RAGN12 |
| 2C TCR transgenic mice | Thomas Gajewski lab | Sha et al., 1988 |
| CD11cDTR/GFP B6.FVB-Tg (ItgaxDTR-EGFP) | Jackson Laboratory | Cat# 004509 |
| Batf3−/− | Jackson Laboratory | Cat# 013755 |
|
| ||
| Oligonucleotides | ||
|
| ||
| Primer XmaI-intron-Va10 forward | IDT | 5′CATCTCCCGGGGCCACACAAG CACCATGAAGAGGCTGCTGTGCTCTCTGC3′ |
| Primer SacII-intron-TRAJ9 reverse | IDT | 5′CACCGCGGTAATTTAAATCAAGT TTCTCATTGCACTCACTTGGA TCAACCAACAAGCTTGTTCCTG3′ |
| Primer XhoI-intron-TRBV5 forward | IDT | 5′AGCCACCTCGAGCCTGATTCCACCATG AGCTGCAGGCTTCTCCTCTATGTTTC3′ |
| Primer SacII-intron-TRBJ1–6 reverse | IDT | 5′CTGCAACCGCGGTCAGAAATGGAGCCCC CATACCTGTCACAGTGAGCCGGGTGCCTG3′ |
|
| ||
| Recombinant DNA | ||
|
| ||
| pTα and pTβ cassettes | Diane Mathis lab | Kouskoff et al., 1995 |
| pLEGFP-N1 | Clontech | 6059–1 |
|
| ||
| Software and algorithms | ||
|
| ||
| Flowjo_V10 | Treestar | Version 10; https://www.flowjo.com/ |
| Prism | GraphPad | Version 8; https://www.graphpad.com/scientific-software/prism/ |
| Adobe Illustrator | Adobe | Version 25.3.1, https://www.adobe.com/ |
|
| ||
| Deposited data | ||
|
| ||
| RNA-Seq | This paper | GEO # GSE186268 |