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. 2021 Nov 25;12:760476. doi: 10.3389/fmicb.2021.760476

FIGURE 3.

FIGURE 3

Anti-inflammatory effect of Limosilactobacillus reuteri EFEL6901 in LPS-induced RAW 264.7 cells (A–C) and LPS-induced mouse peritoneal macrophage cells (D–F). Effects of heat-killed L. reuteri EFEL6901 on the production of NO (A), mRNA expression of iNOS (B) and COX-2 (C) in LPS-induced RAW 264.7 cells were measured after 24 h. Expressed mRNA levels of iNOS and COX-2 were determined by real-time PCR. Effects of heat-killed Lactobacillus reuteri EFEL6901 strain on IL-12 (D), IL-10 (E) and the calculated ratio IL-10/IL-12 (F) in LPS-induced mouse peritoneal macrophages. Cells were treated with heat-killed bacteria and stimulated with LPS for 24 h. The levels of IL-12 and IL-10 in cell culture supernatants were measured by ELISA kits. Different letters indicate a significant difference at p < 0.05 according to Duncan’s multiple range test. Significant differences are presented with LPS positive group: *p < 0.05; **p < 0.01; ***p < 0.001.