Skip to main content
. Author manuscript; available in PMC: 2021 Dec 9.
Published in final edited form as: Cell Rep. 2021 Nov 9;37(6):109965. doi: 10.1016/j.celrep.2021.109965

Figure 4. Mtbp is under positive selection.

Figure 4.

(A) Positive selection signals in Mtbp gene. We observed nucleotide positions and indels under positive selection in the coding sequences of 3 exons, corresponding to the mid-domains and the C domains of Mtbp.

(B) Nucleotide sequence changes between the beaver and human genomes. We predicted the deleteriousness of these changes based on their human genome annotation, using the following metrics and thresholds: PROVEAN score < ‒2.5, 0.85 < PolyPhen2 score < 1.0, and CADD score > 20. In addition, the change of S→W (AGT→TGG) is a splicing variant, 1 bp away from the splicing junction.

(C) Mtbp gene expression in tissues of beavers and mice. *FDR < 0.01; **adjusted p < 0.01 (Bonferroni correction). Five biological replicates for beaver liver and skin tissues; 4 biological replicates for beaver brain, kidney, and spleen tissues. Five biological replicates for each tissue of mouse. Data are shown as mean ± SD.