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. 2021 Dec 5;13(23):6124. doi: 10.3390/cancers13236124

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Sorting of pancreatic cancer stem cells and tumorigenic activity. (a) Following disaggregation of PANC-1-derived pancreatic cancer spheres, CD44⁺CD24⁺EpCAM⁺ CSCs were purified by means of flow cytometry sorting as described in the Section 2. The percentage of the cells corresponding to the CSC population is indicated in the pre-sorting (Presort) and post-sorting (Postsort) histograms. (b) In immunodeficient mice, 10², 10³ and 10⁴ PANC-1 cells and freshly sorted CD44⁺CD24⁺EpCAM⁺ PANC-1 CSCs were injected, and tumor development was analyzed after 4 months. Following 17 weeks, the animals were sacrificed and the weight and volume of each tumor generated in the animals were determined, and the corresponding average values were presented. The data are the means ± SD (n = 10); p values represent the statistically significant difference in the generated tumor size and weight between the mice injected with PANC-1 cells and CD44⁺CD24⁺EpCAM⁺ PANC-1 CSCs; *, p < 0.05; **, p < 0.01.