FIG. 7.

Effect of IRS protein overexpression on Akt phosphorylation in embryonic fibroblast cell lines. After overnight serum starvation, cells were stimulated with 10 nM IGF-1 for 10 min at 37°C. Cell lysates were subjected to SDS-PAGE and analyzed by Western blotting with anti-phospho-Akt antibody (Ser473). Blots were visualized by autoradiography (upper panel) and quantified by scanning densitometry (lower panel). Quantitative results, expressed as percentages of IGF-1-induced WT cells, are presented as the mean and SEM of three independent experiments.