Table 3.
Identification of 40s small ribosomal proteins (RPS) and 60s large ribosomal proteins (RPL) that interact with PGRMC1 in Ishikawa endometrial cancer cells. Flag antibody coated magnetic beads were used to pull-down PGRMC1-FLAG:protein complexes in PGRMC1-FLAG expressing cells. The list of proteins is based on matched peptides identified using Proteome Discoverer software with each protein having at least 10% protein coverage and two or more unique peptides assigned to each protein.
| Ribosomal Protein Interaction with PGRMC1 (% coverage) | |||
|---|---|---|---|
| RPS25 (30%) | RPL22 (30%) | RPS12 (54%) | RPL4 (24%) |
| RPS4X (40%) | RPS10 (15%) | RPS18 (20%) | RPS20 (19%) |
| RPS14 (32%) | RPL7A (28%) | RPS19 (51%) | RPSA (23%) |
| RPS17 (56%) | RPL5 (22%) | RPS23A (41%) | RPS3 (19%) |
| RPS15A (18%) | RPL18 (49%) | RPS13 (35%) | RPS11 (42%) |
| RPL13A (11%) | RPL38 (47%) | ||