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Expansion of ex vivo of circulating tumor cells (CTCs) derived from patients with HNC. (A) Representative bright-field images of organoids expanded ex vivo from CTCs derived from patients with HNC. Scale bar: 100 μm. (B) Epithelial cell adhesion molecule (EpCAM), or CD45 immunostaining, and Hoechst staining results for organoids to ascertain the presence of CTCs. Scale bar: 100 μm.
