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Validation of seven gene subset using qRT-PCR. Real time qRT-PCR validation of genes of CDC20, CDKN2A, CTSV, FOXM1, KRT23, MAGEA6 and S100A9. The qRT-PCR data was analyzed using REST 2009 © (Relative Expression Software Tool), Qiagen, Germantown, MD, USA. Bar represents the standard error mean (SEM) for three biologicals and three technical replicates.
