Figure 6.

Analysis of pre-clinical in vitro model and their main applications. (A) Most used techniques to analyze in vitro models.; (B) Choice of cell culture models for application in the various downstream applications. Most commonly used in vitro models used for proliferation/migration/invasion assays, drug screening analysis, angiogenesis assay, cellular uptake/release assays, immune cell response assays, extracellular vesicles in vitro functional assay and gene manipulation assays. Bars represent the percentage for each option, with respondents being able to choose more than one option as appropriate. Percentages were calculated based on the reported use of each technique.