FIGURE 1.

Connexin 46 (Cx46, green) and phalloidin (F-actin, red) staining in cross sections from control, EphA2^–/– and ephrin-A5^–/– lenses. Images are from the periphery to the inner mature fiber cells of the lens. (A) Cx46 is present at the cell membrane and is enriched on the long sides of peripheral differentiating fiber cells and becomes uniformly distributed around the cell membrane during maturation. The staining pattern is similar between control and EphA2^–/– lens fibers, despite the disorganization of EphA2^–/– cells. (B) Cx46 displays a similar localization pattern in peripheral and mature control and ephrin-A5^–/– lens fibers. Scale bars, 20 μm.