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. 2021 Nov 25;13(23):5937. doi: 10.3390/cancers13235937

Figure 5.

Figure 5

Orai3 contributes to Panc1 cell-cycle progression. (A,B) Representative data showing FACS-based cell-cycle analysis of Panc1 shNT and Panc1 shOrai3 stable cells. Cell-cycle analysis was performed with three independent biological experiments using propidium iodide. (C) Quantitative analysis of % cells in different phases of cell cycle in the case of Panc1 shNT and Panc1 shOrai3 stable cells (n = 4). (D) Western blot analysis for cyclin D1 expression in shNT and shOrai3 Panc1 stable cell lines. (E) Densitometric analysis of cyclin D1 levels in shNT and shOrai3 Panc1 stable cells. (F) Western blot analysis for Cdk4 expression in shNT and shOrai3 Panc1 stable cell lines. (G) Densitometric analysis of Cdk4 levels in shNT and shOrai3 Panc1 stable cells. Data presented as mean ± S.E.M. Unpaired Student’s t-test was performed for statistical analysis. p-value < 0.05 was considered as significant and is presented as “*”.