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. 2021 Nov 23;13(23):5884. doi: 10.3390/cancers13235884

Figure 2.

Figure 2

Dichotomous role of CBP and p300 in cellular metabolism and energetics. (A–C) Bar graph shows the proportion of ATP generated from mitochondrial OXPHOS or glycolysis, as determined by Seahorse ATP rate assay, in MDA-MB-231 control (Ctrl), CBP KD and p300 KD cells (A); in P19 WT and EDITED cells treated with ATRA (B); and in MDA-MB-231 control (Ctrl) and 10 µM ICG-001 treated cells (C). All significance testing was performed compared to control. (D) Bar graphs display details about mitochondrial respiratory capacities for MDA-MB-231 control (Ctrl), CBP KD and p300 KD cells. (E) Bar graphs show details regarding mitochondrial respiratory capacities for P19 WT and EDITED cells treated with ATRA. Glycolytic capacities are shown in bar graphs for MDA-MB-231 control (Ctrl), CBP KD and p300 KD cells (F), and P19 WT and EDITED cells treated with ATRA (G). (H) Cell growth rate assays for MDA-MB-231 control (Ctrl), CBP KD and p300 KD cells cultured in different media, including regular DMEM medium, DMEM with 2-DG and galactose medium. All significance tests were performed compared to control. * p < 0.05, ** p < 0.01, *** p < 0.001.