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. 2021 Nov 23;13(23):5884. doi: 10.3390/cancers13235884

Figure 4.

Figure 4

Differential roles of CBP and p300 in mitochondrial biogenesis and activity. (A) Immunofluorescence staining for TOM20 in MDA-MB-231 cells (left panel) and quantification of the percent of cells containing tubulated, intermediate and fragmented mitochondria (right panel). At least 100 cells were quantified for each cell line. ** p < 0.01, n.s. not significant. (B) Expression of the mitochondrial membrane protein TOM20 was examined in MDA-MB-231 control (Ctrl), CBP KD and p300 KD cells. (C) Transmission electron micrographs of the ECM show mitochondrial morphology in MDA-MB-231 cells. Red arrows indicate elongated mitochondria in CBP KD cells. (D) Mitochondrial activity and mass were investigated by FACS utilizing MitoTracker Orange and MitoTracker Green in P19 WT and P19 p300 edited (EDITED) cells treated with ATRA. (E) P19 WT and P19p300 edited (EDITED) cells were cultured in Glucose (Glu) or Galactose (Gal) medium. The mitochondrial activity and mass were investigated via MitoTracker Orange and MitoTracker Green by FACS. (F) Relative mRNA expression levels were examined for mitochondria related genes, including SAMM50, TSPO, AARS2, HEBP2 and MFN2. * p < 0.05, ** p < 0.01, *** p < 0.001.