Figure 3.

Spaghetti plots of integrin αvβ6 expression within VSCC patients. Lines in this plot represent patients, a dot is an average H-score of a vulvar tissue type within that patient’s tissue section (for instance the average H-score of all adjacent HSIL tissue annotations). Left: HPV-independent VSCC patients, right: HPV-dependent VSCC patients. Based on H-scores, TBRs are calculated (H-score (pre)malignant tissue/H-score of healthy tissue = TBR). A green line indicates higher expression of αvβ6 in (pre)malignant compared to the healthy tissue (TBR > 2); an orange line indicates a TBR < 2 for VSCC and a TBR > 2 for HSIL compared to healthy tissue; a red line indicates higher expression in healthy compared to (pre)malignant tissue (TBR ≤ 1). Not all TBRs of VSCC patients are plotted, as not all tissue samples included normal (and/or premalignant) tissue adjacent to the tumor.