FIG. 4.

PCR-based identification of Bartonella species from animals known to be infected with either B. clarridgeiae (sample A), B. henselae (sample B), or B. vinsonii subsp. berkhoffii (sample C). DNA was extracted from 200 μl of blood and was eluted in a final volume of 200 μl, and then 5 μl of template DNA was used in each PCR amplification. After amplification, the PCR products were electrophoresed on a 3% agarose gel and stained with ethidium bromide. Amplified control template DNA derived from isolated B. clarridgeiae, B. henselae, and B. vinsonii subsp. berkhoffii strains yielded expected products of 154, 172, and 260 bp, respectively. The first and last lanes each contain a 20-bp ladder.