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Apoptosis analysis with flow cytometry using Annexin V-FITC in U-87 cell line at 24 h (upper row) and 30 h (lower row) time points, where groups are: (a,a1) untreated: UT (b,b1) temozolomide: TEMO, positive control, and (c,c1) AgZE (IC50: 4.1 μL). AgZE treated U-87 cells exhibits increase in cell population in early apoptotic phase at 24 h which later moves to late apoptotic region at 30 h.
