Figure 2.

TH588 induces severe chromosome congression problems that lead to mitotic arrest, followed by the cell death or erroneous cell division. (A) Representative spinning disk confocal time-series of mitosis in U2OS cells stably expressing H2B-GFP/mCherry-α-tubulin undergoing indicated treatments. Scale bar 10 µm. (B) Quantification of duration of mitosis in U2OS cells undergoing indicated treatments. Scatter plot graphs with median are plotted from three independent experiments (n = 56 cells for DMSO, 49 cells for 5µM TH588, 63 cells for 100 nM nocodazole, and 65 cells for 20 nM nocodazole). (C) Quantification of the fate of mitotic cells from three independent experiments. **** p < 0.0001.